4种不同性诱剂对玉米草地贪夜蛾诱集作用

为准确掌握草地贪夜蛾成虫发生动态,筛选对当地种群专一性强、灵敏度高的性诱剂,对深圳百乐宝生物农业科技有限公司(BLB)、宁波纽康生物技术有限公司(NK)、江苏宁录科技股份有限公司(NL)、南京新安中绿生物科技有限公司(XAZL)4个厂家生产的草地贪夜蛾性诱剂在江苏盐城市大丰区进行田间诱捕效果试验,探讨草地贪夜蛾性诱剂的使用技术。结果表明:4种不同性诱剂诱芯对草地贪夜蛾的诱集效果存在明显差异。BLB诱芯和NL诱芯可以有效监测草地贪夜蛾成虫田间发生动态,羽化高峰期明显,且峰值日期一致,NK诱芯诱集的成虫虫峰不明显,XAZL诱芯零星诱到成虫,无明显成虫高峰;BLB诱芯监测期内日均诱蛾量、最高单次诱蛾量、总诱蛾量均显著高于其他3种诱芯,其总诱蛾量达136.75头,分别是NL诱芯、NK诱芯、XAZL诱芯的1.47倍、4.76倍、10.52倍;通过对BLB诱芯进行持续30 d和持续60 d的诱捕对比试验,发现BLB诱芯1~30 d内诱捕性能稳定,诱蛾量大,31~60 d内日均诱蛾量、最高单次诱蛾量、总诱蛾量均有所下降且达显著水平,尤其是50 d后诱捕量下降明显。结果表明:BLB诱芯的诱蛾量大,能够明显反映出草地贪夜蛾的羽化高峰期,符合测报要求,更适合本地区草地贪夜蛾的监测,使用该诱芯时建议30 d更换1次。     

生长环境及收获期对野鸦椿属植物三萜类化合物含量的影响

【目的】研究不同生境和收获时间对圆齿野鸦椿不同部位总三萜类化合物积累的影响情况,为其药用价值和科学栽培的研究奠定基础。【方法】选择3个低海拔地区的8年生圆齿野鸦椿,分别于2014年11月和2015年3月采集其叶片、枝条和果实,选择高海拔地区6年生的野鸦椿和圆齿野鸦椿,于2014年8月分别采其叶片和枝条,对其总三萜类化合物含量进行了测定和比较分析。【结果】1)8月采集的叶片中三萜类化合物的含量,野鸦椿高于圆齿野鸦椿,且其含量差异呈显著性水平;但是,8月采集的枝条中三萜类化合物含量,野鸦椿低于圆齿野鸦椿。2)圆齿野鸦椿不同部位的总三萜类化合物含量存在差异,且2个月份采集的含量高低顺序均为叶片>枝条>果皮>种子,均以叶片中的含量为最高,依次为9.06%(农大)、7.14%(清流)和6.85%(邵武)。3)11月采集的圆齿野鸦椿样品中的三萜类化合物含量高于3月所采样品中的含量,其叶片中的总三萜类化合物含量最多可提高172.91%。4)圆齿野鸦椿叶片、果皮和种子中的三萜类化合物含量与试验地的海拔高度、年降水量均表现出极显著的负相关关系,与年均气温存在极显著的正相关关系,其叶片中的三萜类化合物含量还与年均无霜期呈正相关关系,其枝条中的三萜类化合物含量与各地理和气象因素之间的相关性均不显著。【结论】圆齿野鸦椿的叶片采收期长于野鸦椿;低海拔、较少降水量和年均气温较高有利于圆齿野鸦椿植株对三萜类化合物的累积,这3个因子可以作为圆齿野鸦椿适宜栽培区域的划分指标;夏季采摘野鸦椿叶片,秋冬季采摘圆齿野鸦椿叶片,所采叶片的总三萜类化合物的含量均较高。     

玉米开花期相关的Indeterminate domain(IDD)蛋白家族基因的鉴定

开花期是影响玉米产量的重要因子之一。Indeterminate1 (ID1)编码玉米Indeterminate domain (IDD)家族蛋白,是玉米开花期的重要调控因子。然而,其他玉米IDD蛋白家族基因及其生物学功能有待深入研究。本文利用生物信息学技术在玉米基因组中鉴定并分离了37个IDD家族基因,记作ZmIDD。表达分析发现这些ZmIDD基因在8个玉米组织中显示出多种表达模式。为进一步探讨ZmIDD基因在调控玉米开花期上的作用,检测了37个ZmIDD在172个自交系中的遗传多样性,发现35个ZmIDD基因在自交系间具有多态性,平均每个基因具有37.8个多态性位点。关联分析鉴定到包含ID1在内的7个ZmIDD基因在多个环境下与开花期性状显著关联。对Zm00001d020683基因2 kb的启动子区和600 bp编码区重测序,共鉴定到64个多态性位点。候选基因关联分析鉴定到2个启动子区的插入缺失(In/Del)位点与开花期显著关联,其中2个位点分别插入3 bp和2 bp的单倍型为一种提早开花的基因型。研究结果为玉米开花期相关基因的分离和利用研究提供了候选基因和选择靶点。     

应用缩节安(DPC)调控棉花株型的定位定量效应研究

缩节安(1,1-dimethyl piperidinium chloride, DPC)是棉花生产中广泛应用的植物生长延缓剂,其调控棉花茎枝生长的定位定量效应尚缺乏系统的量化研究。本研究2013—2014年在田间条件下分别于棉花现蕾期、盛蕾期后、盛花期前、盛花期后和打顶后单次应用不同剂量的DPC,测量了DPC作用有效期内的棉花株高和主茎生长速率,探究了所有主茎节间及所有果节对DPC的响应。结果表明, DPC处理对棉花主茎节间的影响范围为N节(应用DPC时的主茎节)以下1～4个和N节以上0～6个(打顶条件下),对果枝的影响范围为N节以下1～11个和N节以上0～5个,其中N节以下果枝受影响的果节多于N节以上果枝。将盛蕾期后和盛花期前2次应用DPC的效应叠加,其影响范围几乎可以覆盖全部主茎节间(果枝始节以上)和全部果节。DPC应用剂量与其作用强度并不总存在较好的线性关系。DPC的定位定量效应除了与应用时间和剂量有关,还受到温度、降水等环境条件和棉株生物量、源库关系的影响。     

水稻OsUBA基因的表达及其在促进种子萌发和开花中的功能

自噬是将功能异常或不需要的胞内组分降解的细胞学过程,广泛参与真核生物的生长发育过程、对营养缺乏的响应及生物/非生物胁迫反应。NBR1 (Next to BRCA1 gene 1, NBR1)是在植物中发现的最重要的自噬受体,但有关植物NBR1类自噬受体的研究较少,水稻中此类蛋白的研究还是空白。本文通过RT-PCR方法,从水稻日本晴幼苗的cDNA中克隆到一个含有泛素相关结构域(Ubiquitinassociated,UBA)的基因,将其命名为OsUBA。OsUBA的开放阅读框长2538 bp,编码845个氨基酸残基。OsUBA属于水稻中的NBR1类蛋白。OsUBA的启动子区有多个与光、逆境胁迫及激素反应相关的元件; OsUBA基因在水稻花药、正在萌发的种子以及根中的表达量较高,在茎和叶中也有表达; 200μmol L~(–1) ABA处理显著抑制OsUBA的表达,100μmol L~(–1) GA处理后OsUBA的表达略有升高。对OsUBA过表达水稻株系的研究表明,转基因水稻种子的萌发比野生型更快, ABA (3μmol L~(–1))处理显著抑制OsUBA过表达水稻株系种子的萌发, GA (100μmol L~(–1))处理对OsUBA过表达水稻株系种子的萌发略有促进;OsUBA过表达水稻株系的开花时间较野生型明显提前。这些结果表明,水稻NBR1蛋白基因OsUBA的表达和功能可能与对开花时间和种子萌发的调控以及生物/非生物胁迫反应有关。     

Diversity in daytime and night‐time transpiration dynamics in barley indicates adaptation to drought regimes across the Middle‐East

A challenge to breeding drought‐tolerant barley in the Middle‐East is that precipitation and evaporative demand patterns dictate opposite water use strategies (conservative vs. risk‐taking). To characterize these strategies, we examined high‐resolution, whole‐plant transpiration rate (TR) responses to increasing vapour pressure deficit (VPD) and nocturnal TR (TR_N) dynamics among 25 local barley genotypes, using a novel phenotyping system. These traits were specifically selected because they exist under modalities enabling the expression of both strategies. The genotypes were selected from locations spread across a large aridity gradient represented by temperature and precipitation data spanning 30 years. Here, we uncovered a substantial diversity in TR responses to VPD where slopes of the linear responses correlated negatively with local maximal temperatures, pointing to opposite drought tolerance strategies. Low canopy conductance (low slopes) was associated with higher aridity, likely to enable water‐saving, while higher conductance was associated with wetter areas, likely to enable a more aggressive water use to maximize physiological activity. TR_N was highly diverse and represented up to 15% of maximal daytime TR, pointing to the possibility of increasing water‐saving by reducing TR_N. Furthermore, we detected pre‐dawn variation in TR_N that negatively correlated with local precipitation, indicating that a tighter circadian control is associated with adaptation to drought, consistently with a circadian resonance mechanism. These findings indicate that canopy conductance and TR_N are potentially beneficial to design drought‐tolerant barley germplasm adapted to different drought regimes taking place in the Middle‐East.     

Some Agronomic Strategies for Organic Quinoa (Chenopodium quinoa Willd.)

Quinoa is a potential new seed crop for protein feed and human consumption in Europe, with tolerance to a range of abiotic stresses. For this purpose the study was planned to analyse the effect of important agronomic strategies like nitrogen level, N application strategy, row spacing and harvest time on yield and quality of quinoa. The experiments took place in the field of the experimental station of the Faculty of Science, University of Copenhagen. Three levels of organic nitrogen from slurry was used (60, 120 and 180 kg N ha^?1), supplied either all at once at sowing, or split between sowing and beginning of the reproductive phase. The effect of row spacing and harvest time was studied by harvesting seeds at seed maturity, which occurred 2–3 weeks prior to the mechanical harvest by threshing, and a couple of months after. Yield increased significantly (P ≤ 0.05) with an application up to 180 kg N ha^?1, reaching 2200 kg ha^?1. Increasing N also caused a significantly increased seed weight (up to 3.3 mg) and protein content (up to 17 %). N level did not affect number and amount of weeds. Split application with part of the N applied at bud formation did not have a significant effect on yield. Delayed harvest had a negative influence on seed weight, whereas protein content was stable after harvesting even a month after seed maturity. A late harvest significantly reduced seed germination, being reduced by 50 % after a 2‐month delay. A conclusion from this study is that both yield and protein content of seed can be manipulated by N level and application strategy. Harvest time is important for securing a high seed quality measured as seed germination, seed weight and protein content. A fast germination of quinoa is an important characteristic demonstrating that the crop has good possibilities for being well‐established in the field when free from weeds at the time of sowing. The choice of row spacing is important and depends on weed control method. Weed control strategy should be developed based on modern precision tools.     

靶向S基因外源性microRNA抑制TGEV增殖效果的研究

应用生物信息学软件对猪传染性胃肠炎病毒(TGEV)S基因进行分析,筛选出可能与S基因有相互作用的外源miRNA:amiRNA-S-28035,amiRNA-S-28038,amiRNA-S-28165。之后,利用脂质体将构建好的相应的表达载体瞬时转染至PK-15细胞;通过Q-PCR和间接免疫荧光方法检测其对S基因的抑制作用;CPE分析和TCID50测定检测其对TGEV增殖的抑制效果。结果发现,3个外源性miRNA均降低了S基因mRNA的转录和蛋白的表达,其中amiRNA-S-28038对TGEV mRNA的平均抑制率可达64.6%,最高可达69.9%,表明外源性microRNA可以通过靶向TGEV基因组来抑制TGEV的复制。研究结果为猪传染性胃肠炎的预防和治疗提供了新的思路。     

减少底肥施用量增加叶面施肥次数对玉米产量的影响

该试验目的是研究降低底肥施用量但是增加叶面肥施用次数方面对玉米产量的变化规律，旨在找出最经济的施肥方式以达到在保护环境的基础上提高经济效益的目的。试验表明，玉米产量随着底肥施用量的增加而提高，并且叶面肥喷施次数越多产量越高，每喷一次，可增加产量258~592.5 kg/hm2，增产幅度2.3%~5.6%。     

关税和补贴对国企国有股份最优占比的影响——基于混合寡占理论分析

通过建立理论模型，并用2014年湖南省5县区20个城乡社区的调查数据，从家务劳动时间的视角分析湖南劳动力市场的性别工资差异及其影响因素。结果发现，家务劳动时间是影响湖南劳动力市场性别工资差异的重要因素，同时其他影响因素还有月工资收入、受教育程度、行业等，但职务职称对性别工资差异的影响存在不确定性。因此，政府应大力发展家庭服务业，建立健全覆盖城乡的家庭服务体系，基本满足家庭的服务需求，使女性从家务劳动中解脱出来，同时应通过政策倾斜确保女性培训比例，增强就业竞争实力；规范劳动合同，维护私有及民营企业女性从业者在劳动就业、社会保障等方面的合法权益。